Microscope magnification: 10X, 20X – does it matter what it says on the tin?

What does 10x magnification or 20x magnification mean in digital microscopy?

Magnification differences between digital and conventional microscopy

In conventional light microscopy, the magnification of the objective lens is very straightforward: a 20x magnification means the image is magnified to a size 20 times larger than the original. Thus, when viewed with 40x magnification, objects seem twice as big as with a 20x lens. It’s logical that the pathologist wants a turret of objectives with a range of magnification to be able to get an overview of the sample, to examine it and to make an informed diagnosis. It also seems logical to think a higher magnification objective is “better”. But does it matter what it says on the tin?

In digital microscopy the magnification steps are achieved in a different manner. The slide is scanned, i.e. photographed in full resolution and the resulting image can be zoomed in and out to get the best scale view of the sample. The simplicity of the continuously variable magnification of a digital zoom makes moving between an overview and the desired level of detail incredibly easy. Digitally, any part of the image can also be magnified to a size far larger than it would ever appear through an eyepiece. A good overview camera and a single, high quality objective lens are thus enough to make an excellent digital microscope which is easy and fluent to use.

Ocus40 microscope slide scanner with 40x magnification

The Grundium Ocus digital microscope scanners

The Grundium Ocus delivers the highest quality images in digital pathology. The Ocus uses color stacking as one of the steps to achieve this. In conventional RGB imaging, the color channels are mapped in a single image, resulting in an approximation in assigning color value to each pixel. The Grundium Ocus takes full-resolution photos individually of each color, red, green, and blue, and the final image is automatically composed of these three layers. This process results in a sharp image with a far superior level of detail, as the starting point is three times more pixels than when using a conventional color sensor.

Where other scanners sweep the sample using a pre-defined focus map, the Grundium Ocus automatically focuses each field, putting the whole sample in focus without making time-consuming focus maps.

In summary, the Grundium Ocus is a combination of optimal magnification and optimal numerical aperture. It quickly produces a clear and sharp scanned image, which is excellent for digital pathology. The scanned images are effortless to view and zoom on a screen. The images or even remote operation of the Ocus scanner can be swiftly shared online. Ease of sharing means an expert, a group of students, a lecture audience, or a second opinion can be available just a couple clicks away.

Two scanners, two magnification levels

Both of the Ocus® series microscope scanners are single slide whole slide scanners (WSI scanners) optimized for their specific tasks, but each masters a broad spectrum of pathology work. They look almost the same and boast the same excellent features: the small footprint, ease of use, superior connectivity, etc. – but the difference is in their comparable magnification levels.

The Ocus®20, with a comparable magnification of 20x, is optimized for fast and effortless scanning. Its perfect balance between image resolution and scan time makes it especially suitable for intraoperative frozen sections and histopathology.

The Ocus®40, with its comparable magnification of 40x, is designed to scan images at a high resolution, which makes it suitable for a wide range of applications, such as cytology.

Book an online demo

Choosing the best setup in digital microscopy is different from traditional light microscopy. Book an online demo and see for yourself how easy it is to scan and share images with the Ocus, and how it fits your line of work. You’ll be surprised how this image quality and usability level can come in such a small, affordable, and beautifully designed package!

If you have questions about picking the right setup for your application, book an online demo with our experts!

More about digital magnification

Read an in-depth description of digital magnification by Grundium's Principal Imaging Engineer Markus Vartiainen: Resolution and scanning speed in high-power digital microscopy.

About Grundium

A global leader in advanced imaging technology, Grundium makes digital pathology and best professional diagnosis available for all life – whether human, animal, plant or other. This is achieved by doing something that nobody else can: applying state-of-the-art mobile technology in digital pathology. Established in 2015 by ex-Nokia engineers, the Tampere-based company is democratizing digital pathology with the Ocus® microscope scanners. The cutting-edge imaging solutions are based on over 20 years of experience in optics, sensors and beautiful high-precision devices. Grundium serves various industries and businesses enhancing quality and processes, protecting human life and safeguarding a clean environment.
It is invaluable to be able to ask more experienced colleagues for their opinion and the Ocus would enable me to do it in a matter of minutes from my home office. This is approximately the time it would take me to visit a colleague in the office next-door, only now, I can invite more people from different locations for this digital review at the same time from any location.
— Dr. Alexandra Zuraw
The Digital Pathology Place 

Book a live demo

See the OCUS® portable digital microscope scanner in action – request your free personal online live demo now. A Grundium expert will contact you personally to schedule a suitable 30-minute demo time for you. In the demo we will answer all your questions about which Ocus® scanner fits your needs best, we will talk pricing, share stories about how others are using the scanners, and help you fast forward the digital pathology experience in your lab.

Book your demo